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Table 1 Comprehensive list of virus-induced NET formation in vitro and in vivo

From: C-type lectins and extracellular vesicles in virus-induced NETosis

Virus

In Vitro

In Vivo

 

Species

Origin of neutrophils

Induction of NETs

Induction time (hour)

Detection of NET structure

Species

Induction of NETs

Induction time

Occurred organs

Detection of NET

structure

Ref

Influenza A virus (IAV)

H1N1 (PR8)

Mouse, BALB/c

BALF from uninfected BALB/c mice

Cocultured with influenza- primed LA-4

cells

2.5

IFa

Mouse, BALB/c

500 PFU

(i.n.)

6 days post- infection

Lung

H&E

12

IFb

Mouse,

C57BL/6

BM-derived

neutrophils

BAFL from

infected mice

0.5

IFc

Mouse,

C57BL/6

500 PFU

(i.n.)

5 days post-

infection

Lung

IFc

13

H3N2

(Phil82)

Human

Peripheral blood

MOI = 50

3

SYTOX

Green

N/A

N/A

N/A

N/A

N/A

30

Human immunodeficiency

virus-1 (HIV-1)

Human

Peripheral blood

Pseudotyped HIV-1

(1–6 ng/ml)

24

Hoechst

N/A

N/A

N/A

N/A

N/A

15

Myxoma virus (MYXV)

N/A

N/A

N/A

N/A

N/A

C57BL/6

mice

1 × 107 PFU

(i.v.)

8 h post infection

Liver

Intravital images*

19

Respiratory syncytial virus (RSV)

Human

Peripheral blood

MOI = 0.0005–0.005

3

IFd Quant-iT

dsDNA kit

N/A

N/A

N/A

N/A

N/A

17

Hantaan virus (HTNV)

Human

Peripheral

blood

MOI = 1

6

SYTOX

Green

Human

, infected patients

N/A

N/A

Kidney and serum

IFe ELSIAa

18

Dengue virus (DV)

(serotype 2)

           

Mouse

C57BL/6

BM-derived

neutrophils

 

8

        

PL046

Human

Peripheral

blood

MOI = 5

3

IFf

stat1−/−

mice

2 × 105

PFU (i.c./

i.p.)

5 days post infection

Spleen

IFg

20

NGC-N

Mouse

C67BL/6

BM-derived

neutrophils

MOI = 3

        

Severe acute respiratory syndrome coronavirus-2

(SARS-CoV-2)

N/A

N/A

N/A

N/A

N/A

Human, infected patients

N/A

N/A

serum

ELISAb

21

  1. IFa = MPO, phalloidin, DNA; IFb = histone H2B and DNA; IFc = histone H2B, MPO, and DNA; IFd = MPO, elastase, and DNA; IFe = histone, elastase,and DNA; IFf = histone H1, MPO, and DNA; IFg = Cit-Histone H3, MPO,and DNA. ELISAa = dsDNA/histone complex; ELISAb = cell-free DNA, MPO-DNA, and Cit-H3. Intravital images* = histone H2Ax and elastase
  2. NGC-N New Guinea C-N, IF immunofluorescence staining, MPO myeloperoxidase, BALF bronchoalveolar lavage fluid, MOI multiplicity of infection, N/A not available, BM  bone marrow, PFU plaque-forming unit, i.n intranasal injection, i.v intraveseel injection, i.c intracerebral injection, i.p intraperitoneal injection