From: C-type lectins and extracellular vesicles in virus-induced NETosis
Virus | In Vitro | In Vivo | Â | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Species | Origin of neutrophils | Induction of NETs | Induction time (hour) | Detection of NET structure | Species | Induction of NETs | Induction time | Occurred organs | Detection of NET structure | Ref | ||
Influenza A virus (IAV) | H1N1 (PR8) | Mouse, BALB/c | BALF from uninfected BALB/c mice | Cocultured with influenza- primed LA-4 cells | 2.5 | IFa | Mouse, BALB/c | 500 PFU (i.n.) | 6Â days post- infection | Lung | H&E | 12 |
IFb | ||||||||||||
Mouse, C57BL/6 | BM-derived neutrophils | BAFL from infected mice | 0.5 | IFc | Mouse, C57BL/6 | 500 PFU (i.n.) | 5Â days post- infection | Lung | IFc | 13 | ||
H3N2 (Phil82) | Human | Peripheral blood | MOI = 50 | 3 | SYTOX Green | N/A | N/A | N/A | N/A | N/A | 30 | |
Human immunodeficiency virus-1 (HIV-1) | Human | Peripheral blood | Pseudotyped HIV-1 (1–6 ng/ml) | 24 | Hoechst | N/A | N/A | N/A | N/A | N/A | 15 | |
Myxoma virus (MYXV) | N/A | N/A | N/A | N/A | N/A | C57BL/6 mice | 1 × 107 PFU (i.v.) | 8 h post infection | Liver | Intravital images* | 19 | |
Respiratory syncytial virus (RSV) | Human | Peripheral blood | MOI = 0.0005–0.005 | 3 | IFd Quant-iT dsDNA kit | N/A | N/A | N/A | N/A | N/A | 17 | |
Hantaan virus (HTNV) | Human | Peripheral blood | MOI = 1 | 6 | SYTOX Green | Human , infected patients | N/A | N/A | Kidney and serum | IFe ELSIAa | 18 | |
Dengue virus (DV) (serotype 2) | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | |
Mouse C57BL/6 | BM-derived neutrophils | Â | 8 | Â | Â | Â | Â | Â | Â | Â | Â | |
PL046 | Human | Peripheral blood | MOI = 5 | 3 | IFf | stat1−/− mice | 2 × 105 PFU (i.c./ i.p.) | 5 days post infection | Spleen | IFg | 20 | |
NGC-N | Mouse C67BL/6 | BM-derived neutrophils | MOI = 3 |  |  |  |  |  |  |  |  | |
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) | N/A | N/A | N/A | N/A | N/A | Human, infected patients | N/A | N/A | serum | ELISAb | 21 |