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Fig. 1 | Journal of Biomedical Science

Fig. 1

From: Chandipura virus dysregulates the expression of hsa-miR-21-5p to activate NF-κB in human microglial cells

Fig. 1

The regulation of PI3K/AKT pathway through hsa-miR-21-5p in CHPV infected human microglial cells. The human microglial cells were infected with CHPV at MOI 0.1 and were harvested 24 h post infection. A The expression of hsa-miR-21-5p was checked by qPCR using TaqMan primers and probes specific for miR-21-5p. The expression of hsa-miR-21-5p was normalized to the expression of RNU6B and was 1.6 folds upregulated compared to Control. B Western Blot analysis showed downregulation of PTEN expression in human microglial cells. C The graph displays the densitometry analysis of PTEN normalized to β-tubulin and showed significant decrease of 40 % in PTEN expression. D Western Blot analysis showed induction in p-AKT/AKT expression. E The graph of densitometry analysis of p-AKT/AKT showed significant increase of 1.8 folds in p-AKT expression. The experiments were performed in triplicate (n = 3) and shown as SE ± mean. Student unpaired t test has been applied by using GraphPad-prism 8 (ver 8.3.0) to calculate the p-values, where: *p < 0.05, **p < 0.01, and ***p < 0.001

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