Fig. 3

The effect of hsa-miR-21-5p overexpression in microglial cells during CHPV infection. The hsa-miR-21-5p was over-expressed in human microglial cells followed by CHPV infection. A The over-expression of miR-21 was confirmed by qPCR using TaqMan primers and probes specific for hsa-miR-21-5p. The expression of hsa-miR-21-5p was normalized to the expression of RNU6B. The hsa-miR-21-5p increased by 590 folds in hsa-miR-21-5p + CHPV compared to CHPV, while hsa-miR-21-5p was 550 folds upregulated compared to scramble. B Western Blot analysis showed downregulation of PTEN expression after over-expression of hsa-miR-21-5p. C The graph displays the densitometry analysis of PTEN normalized to β-tubulin and showed significant decrease of 20 % in hsa-miR-21-5p + CHPV compared to CHPV, while hsa-miR-21-5p was 30 % downregulated compared to scramble. D Western Blot analysis showed induction in p-AKT/AKT expression post over-expression of miR-21 and infection in human microglial cells. E The graph of densitometry analysis of p-AKT/AKT showed significant increase of 30 % in p-AKT expression in hsa-miR-21-5p + CHPV compared to CHPV, while hsa-miR-21-5p was 30 % upregulated compared to scramble. The experiments were performed in triplicate (n = 3) and shown as SE ± mean. One-way ANOVA has been applied by using GraphPad-prism 8 (ver 8.3.0) to calculate the p-values, where: *p < 0.05, **p < 0.01, and ***p < 0.001