Fig. 5

The effect of hsa-miR-21-5p over-expression on NF-κB activation. The effect of hsa-miR-21-5p on NF-κB activation was studied during over-expression and CHPV infection in human microglial cells. A Western Blot analysis showed induction in pNF-κB/NF-κB expression post over-expression of hsa-miR-21-5p and infection in human microglial cells. B The graph of densitometry analysis of pNF-κB/NF-κB showed significant increase of pNF-κB expression by 45 % in hsa-miR-21-5p + CHPV compared to CHPV, while hsa-miR-21-5p alone was upregulated 60 % compared to scramble. C The expression of IL-6 during hsa-miR-21-5p over-expression and CHPV infection was checked using qPCR. The expression of IL-6 was normalized to the expression of GAPDH. The expression of IL-6 got increased in hsa-miR-21-5p over-expression by 2.1 folds as compared to scramble. In hsa-miR-21-5p over-expression + CHPV, pNF-κBp65 /NF-κBp65 expression was 2.8 folds higher compared to CHPV D The expression of TNF-α 24 h post CHPV infection, was checked using qPCR. The expression of TNF-α was normalized to the expression of RNU6B. The expression of TNF-α got increased in hsa-miR-21-5p over-expression by 1.3 folds as compared to scramble. In hsa-miR-21-5p over-expression + CHPV, pNF-κBp65 /NF-κBp65 expression was 1.5 folds higher compared to CHPV. The experiments were performed in triplicate (n = 3) and shown as SE ± mean. One-way ANOVA has been applied using by using GraphPad-prism 8 (ver 8.3.0) to calculate the p-values, where: *p < 0.05, **p < 0.01, and ***p < 0.001