Fig. 7

NF-κBp65 luciferase promoter activity during hsa-miR-21-5p over-expression and knockdown in human microglial cells. The human microglial cells were transfected with hsa-miR-21-5p and anti-hsa-miR-21-5p and then infected with CHPV and to see if hsa-miR-21-5p regulated the activity of NF-κB promoter. A The over-expression of hsa-miR-21-5p during CHPV infection increased the NF-κB-luciferase activity by 25 % compared to CHPV infected sample. The expression of NF-κB was 10 % higher in mimic alone as compared to scramble. B Whereas the knockdown of hsa-miR-21-5p restored the NF-κB-luciferase activity by about 25 % compared to Cy3 + CHPV. The NF-κB-luciferase activity reduced by nearly 40 % in anti-hsa-miR-21-5p as compared to Cy3