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Fig. 2 | Journal of Biomedical Science

Fig. 2

From: RETRACTED ARTICLE: Downregulating lncRNA NEAT1 induces proliferation and represses apoptosis of ovarian granulosa cells in polycystic ovary syndrome via microRNA-381/IGF1 axis

Fig. 2

NEAT1 binds with miR-381, and IGF1 is the direct target gene of miR-381 in PCOS rats. A The putative complementary binding sites of miR-381 and NEAT1; B Luciferase activity assay verified the targeting relationship between NEAT1 to miR-381; C RNA pull-down assay was used to detect the enrichment of NEAT1 by miR-381; D The putative complementary binding sites of miR-381 and IGF1; E Luciferase activity assay verified the targeting relationship between miR-381 and IGF1; F NEAT1 expression in ovarian tissues of PCOS rats by RT-qPCR; G miR-381 expression in ovarian tissues of PCOS rats by RT-qPCR; H IGF1 mRNA expression in ovarian tissues of PCOS rats by RT-qPCR; I IGF1 protein bands in ovarian tissues of PCOS rats by Western blot analysis; J IGF1 protein expression in ovarian tissues of PCOS rats by Western blot analysis; In A–E, N = 3. In F–J, n = 8. The data are all measurement data, expressed as mean ± standard deviation, the t test was used for statistical analysis between the two groups, and one-way ANOVA and Tukey’s post hoc test were used for statistical analysis among multiple groups. *P < 0.05 vs. the normal group; #P < 0.05 vs. the sh-NC group

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