Skip to main content
Fig. 2 | Journal of Biomedical Science

Fig. 2

From: AUY922 induces retinal toxicity through attenuating TRPM1

Fig. 2

Treatment with AUY922 attenuated TRPM1 expression and dysregulated photoreceptors and RPE cells in the mouse retina. a Representative images of H&E stain and IHC analysis for TRPM1, PNA and rhodopsin expression in mouse eye samples from the AUY922 treatment experiment in Fig. 1b. Scale bar: 25 μm. n = 4. b Representative images of RPE65 and Actin expression, as determined by fluorescence immunohistochemistry and confocal scanning laser microscopy, in mouse RPE flat mounts from the AUY922 treatment experiment in Fig. 1b. Scale bar: 25 μm. n = 4. c Representative images of TUNEL assay and IHC analysis for cleaved caspase-3, GFAP, Trpm1, PNA and rhodopsin expression in mouse eye samples from mice treated with 25 mg/kg AUY922 5qw for 2 w before drug withdrawal for 10 d. Scale bar: 25 μm. n = 4. IS: inner segment; OS: outer segment; ONL: outer nuclear layer; OPL: outer plexiform layer; INL: inner nuclear layer. GCL: ganglion cell layer. The arrow indicates the AUY922-sensitive layer

Back to article page