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Fig. 6 | Journal of Biomedical Science

Fig. 6

From: AUY922 induces retinal toxicity through attenuating TRPM1

Fig. 6

TRPM1 mediated AUY922-induced apoptosis, ROS production and cell growth suppression. a Representative western blot results of 661 W (left) and Mel1617 (right) cells stably expressing either a scrambled shRNA or shRNAs specific for TRPM1. β-Actin was used as a loading control. Quantification was performed for three independent experiments. Data are presented as the mean ± s.e.m. The P values were determined by two-tailed Student’s t-test, * P < 0.05; ** P < 0.01; *** P < 0.001; n.s. = not significant. n = 3. b Representative images of clonogenic growth assays in TRPM1-knockdown cells described in Fig. 6a. Fifteen hundred 661 W cells/well and 3000 Mel1617 cells/well were seeded in 6-well plates for 10 d. n = 3. c Representative western blot results of cells stably expressing either an empty vector or 3xF-TRPM1. β-Actin was used as a loading control. n = 3. Quantification analysis was performed for three independent experiments. Data are presented as the mean ± s.e.m. Significances were determined by two tailed Student’s t-test, n.s. = not significant. d Representative images of clonogenic growth assays in cells stably expressing an empty vector or 3xF-TRPM1, after treatment with the indicated concentrations of AUY922 for 10 d. Five hundred 661 W cells/well and 2000 Mel1617 cells/well were seeded in 6-well plates. n = 3. e Apoptosis analysis was performed in cells stably expressing an empty vector or 3xF-TRPM1 after treatment with the indicated concentrations of AUY922 for 24 h. Data are presented as the mean ± s.e.m. The P values were determined by two-tailed Student’s t-test, *** P < 0.001. n = 3

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