Fig. 5

PAK5 promoted ABCB1 transcriptional activation via Wnt/β-catenin signaling pathway. A The protein levels of ABCB1, ABCG2, LRP and MRP2 were detected via Western Blot assay in Hep3B-SR and HepG2-SR cells compared with their parental. B Fluorescent dye rhodamine 123 (Rho123) as an index to test the P-gp activity in Hep3B-SR and HepG2-SR cells compared with their parental. Fluorescent dye rhodamine 123 (Rho123) as an index to test the P-gp activity in Hep3B cells after transfection with PAK5/Vector, PAK5/WT, PAK5/S573N and PAK5/K478M plasmids. C co-IP assay was performed in Hep3B cells by anti-PAK5 antibodies. co-IP assay was performed in Hep3B and Hep3B-SR cells by anti-PAK5 antibodies. D The protein levels of PAK5, ABCB1, β-catenin and p-β-catenin were detected via Western Blot assay in Hep3B-SR and HepG2-SR cells compared with their parental. E qRT-PCR was conducted to determine the expression of PAK5, ABCB1 and β-catenin in Hep3B-SR and HepG2-SR cells compared with their parental. F The protein levels of PAK5, ABCB1, β-catenin and p-β-catenin were detected via Western Blot assay in Hep3B-SR and HepG2-SR cells after PAK5 knockdown. G The protein level of PAK5, ABCB1, β-catenin and p-β-catenin was detected via Western Blot assay in Hep3B and HepG2 cells after transfection with PAK5/Vector, PAK5/WT, PAK5/S573N and PAK5/K478M plasmids. H The protein levels of β-catenin and p-β-catenin were detected via Western Blot assay in Hep3B cells after the co-transfection of PAK5/Vector, PAK5/WT, PAK5/S573N, PAK5/K478M severally with β-catenin or β-catenin/S675A