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Fig. 1 | Journal of Biomedical Science

Fig. 1

From: Bacterial factors required for Streptococcus pneumoniae coinfection with influenza A virus

Fig. 1

IAV coinfection increased the total bacterial burdens of the mice. A Schematic S. pneumoniae serotype 3 mutant library construction and experimental design of mouse infection model. Intranasal challenge of 8- to 10-week-old female BALA/c mice, with or without inoculation of 250 PFUs RP8 influenza virus for 4 days. Fifty microliters of S. pneumoniae serotype 3 mutant (2.5 × 104 CFU) library pools were used for intranasal inoculation. B The mice were sacrificed 2 days post bacterial infection and the bacterial burdens of the mouse lung was calculated via plate counting (n = 5 in each group). Each symbol represents the bacterial burdens value for an individual animal. Mice pre-challenged with influenza viruses RP8 displayed an increase in the total bacterial burdens in the lung (*, p < 0.01, Mann–Whitney U test). IAV influenza A/PR8 virus, PFU plaque-forming units, CFU colony-forming units, Mock without pre-challenged with IAV

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