Table 1 Primers and plasmids used in this study
From: Bacterial factors required for Streptococcus pneumoniae coinfection with influenza A virus
Primer name | Sequence (5’ to 3’) | Purpose |
|---|---|---|
ribA-flank-F | GTATCATATGCGATACAC | ΔribA |
ribA-flank-R | GAGCTGGCTATCATGATG | |
ribA-inverse-F | TGACTGATTATCCTTTCTGC | ΔribA (iPCR) |
ribA-inverse-R | ATGAACACTTATGAAGGTAATTTAG | |
zmpA-flank-FF | CTCAGAAAAAGGAAAAAATC | ΔzmpA |
zmpA-flank-FR | TCCTAATAGTAAACATATTCCTCCTTGAAA | |
zmpA-flank-RF | TTTCAAGGAGGAATATGTTTACTATTAGGA | |
zmpA-flank-RR | CACGACTGAGAGATAATTCTAA | |
zmpA-inverse-F | TATTCCTCCTTGAAATAAAATTTATATATG | ΔzmpA (iPCR) |
zmpA-inverse-R | TGTTTACTATTAGGAAATAAAG | |
Putative aminotransferase-flank-F | TGACACCGCTTCATTTTTCA | ΔPA |
Putative aminotransferase-flank-R | CTTTCCTTGTTGACCCACAG | |
Putative aminotransferase-inverse-F | CTTTGTCTTATCCTTCTAAG | ΔPA (iPCR) |
Putative aminotransferase-inverse-R | AAATCCAGCCTTCTAGGAG | |
prtA-flank-FF | GAAGGAACCACGACACTGC | ΔprtA |
prtA-flank-FR | TCTATAGCTTTTGTCTTTAATTCCTTACAT | |
prtA-flank-RF | ATGTAAGGAATTAAAGACAAAAGCTATAGA | |
prtA-flank-RR | CATCAACTGAGCCAGAATATTTG | |
prtA-inverse-F | TTTAATTCCTTACATATTTATTTAAAC | Δ prtA (iPCR) |
prtA-inverse-R | GACAAAAGCTATAGAAAAAAATG | |
Psepc-F_EcoRIa | CTGAATTCGAAGATCGATTTTCGTTCGTG | Promoter region sequence of spectinomycin |
Pspec-R_PLP-F_OL | ATCATATTTTCCCATTATTTTGATTAGTACC | |
PA-F_Pspec-R_OL | GGTACTAATCAAAATAATGGGAAAATATGAT | Putative aminotransferase gene |
PA-R_CAT-F_OL | TTCAATCTATATCACTTAACGTTTGGCAAA | |
CAT-F_PLP-R_OL | TTTGCCAAACGTTAAGTGATATAGATTGA | Chloramphenicol |
CAT-R_EcoRIa | GTGAATTCTTATTTATTCAGCAAGTC | |
12133-F | TTGCAAGATAAGATTATCCAG | ΔPA::PA |
12136-R | TTAAACGGATATTCTTTAGAG |
Plasmid | Features | Reference |
|---|---|---|
pDL278 | specr; E. coli-Streptococcous shuttle vector | [47] |
pJET1.2/blunt | Cloning vector | Thermo Fisher |