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Fig. 1 | Journal of Biomedical Science

Fig. 1

From: The cell adhesion molecule TMIGD1 binds to moesin and regulates tubulin acetylation and cell migration

Fig. 1

Identification of moesin as a TMIGD1 binding protein: A Schematic of GST-TMIGD1 cytoplasmic domain and its expression. B Collision-induced dissociation (CID) MS/MS spectrum assigned to the C-terminal moesin peptide QRIDEFESM[O] (precursor [M + 2H]2+ m/z 585.578, one of four high-confidence moesin peptide matches for analysis of the tryptic digestion products of xx. *Indicates oxidation of methionine; blue diamond indicates the residual precursor ion. C Whole cell lysates from HEK-293 cells expressing GFP tagged moesin or control vector (EV) were incubated with GST or GST-TMIGD1 coated beads. The captured proteins were released and resolved on SDS-PAGE followed by Western blotting using an anti-GFP antibody. D Whole cell lysates from 786-0 cells expressing EV or TMIGD1 were incubated with anti-TMIGD1 antibody and immunoprecipitated proteins were detected via Western blot analysis using an anti-moesin antibody. The same membrane was blotted for TMIGD1. Whole cell lysates (WCL) from the same group were blotted for total Moesin or GAPDH. E Schematic of GST moesin constructs encompassing the N-terminal GST-FERM or the C-terminal GST-C-ERMAD domains. Coomassie Blue stain of GST, GST-FERM and GST-C-ERMAD in Whole Cell Lysate (WCL) or pulled-down proteins. F Whole cell lysates from HEK-293 cells expressing EV or TMIGD1-Myc were incubated with GST, GST-FERM or GST-C-ERMAD followed by Western blot analysis using anti-Myc antibody

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