Skip to main content
Fig. 4 | Journal of Biomedical Science

Fig. 4

From: The cell adhesion molecule TMIGD1 binds to moesin and regulates tubulin acetylation and cell migration

Fig. 4

Moesin stimulates and TMIGD1 inhibits filopodia protrusions and cell migration. A HEK-293 cells and HEK-293 cells expressing TMIGD1 were transfected with Life-Act7. Filopodia protrusions observed under an epifluorescence microscope after 48 h transfection. Image magnification, 50 µM. White arrows point to filopodia-like protrusions. B RKO cells expressing empty vector or TMIGD1 were stained with phalloidin for actin (red) and DAPI (nucleus). Image magnification, 50 µM. White arrow points to filopodia-like protrusions. The graph is a representative of filopodia formation in RKO cells expressing an empty vector (EV) or TMIGD1 (20 cells/group) **p < 0.01. Cell lysates derived from RKO cells expressing empty vector or TMIGD1 were blotted for TMIGD1 and protein loading control, GAPDH. C RKO cells and RKO cells expressing TMIGD1 were transfected with moesin-GFP. Moesin-GFP localization observed under an epifluorescence microscope after 48 h transfection. Scale bar, 50 µM. The graph is a representative of filopodia formation (based on the localization of moesin-GFP) in RKO cells expressing moesin-GFP (MSN-GFP) alone or co-expressing MSN-GFP with TMIGD1. **p < 0.01. D The same cell groups were subjected to migration assay (quadruples/group) via Boyden chamber assay **p < 0.01. ***p < 0.001. E Western blot analysis of CRISPR-Cas9 mediated knockout of moesin in RKO cells and RKO cells expressing TMIGD1. F Cells were subjected to cell migration assay (quadruples/group) via Boyden chamber assay system *p < 0.05, ***p < 0.001

Back to article page