Fig. 4

Characterization of a spontaneous HPV16-E6/E7-expressing oral tumor model in HLA-A2 (AAD) transgenic mice. A Schematic diagram of the four DNA constructs encoding HPV16 E6/E7, Ras, and SB100 DNA used for tumor model. B Schematic diagram of the experimental procedure. Six to 8 week old HLA-A2 (AAD) transgenic mice (6 per group) were injected with anti-CD3 monoclonal antibody via intraperitoneal (i.p.) injection three times (150 µg/mouse). On the fourth day, mice were injected submucosally (s.m.) with the DNA constructs encoding Ras, SB100, and HPV16E6/E7 (10 µg/plasmid, 30 µl/injection per mouse) into the oral area followed by electroporation. Mice were monitored for tumor growth via IVIS imaging every week for 4 weeks. C Bioluminescence imaging of tumor bearing mice. D Line graph summarizing the bioluminescence imaging of the tumor growth over time. E Kaplan–Meier survival curve of mice bearing HPV16-E6/E7-expressing oral tumor