Fig. 1
From: HBx facilitates ferroptosis in acute liver failure via EZH2 mediated SLC7A11 suppression
Ferroptosis is activated in D-GalN-induced hepatocyte injury model. A Primary hepatocytes were treated with 50 mM D-GalN. Cell viability was monitored by MTT assay. Primary hepatocytes were treated with 50 mM D-GalN or 10 μM erastin for 6 h. B MDA, C intracellular GSH, andD relative iron levels were assessed. E The mRNA levels of GPX4, SLC11A2, SLC7A11 and ACSL4 were determined by qRT-PCR. (F and G) The protein levels of GPX4, SLC11A2, SLC7A11 and ACSL4 were determined by western blot. Primary hepatocytes were treated with 50 mM D-GalN or 50 mM D-GalN + 1 μM Fer-1 for 6 h. H Cell viability, I MDA, J GSH and K relative iron levels were assessed. *, P < 0.05, **, P < 0.01, ***, P < 0.001