Fig. 4

EZH2 inhibition or SLC7A11 overexpression reverses the effects of HBx on D-GalN-induced ferroptosis. Transduced cells were treated with 50 mM D-GalN in the absence or presence of GSK126 (5 μM)/SLC7A11 overexpression vector (MOI = 1, 5, 10) for 48 h. A The mRNA level of SLC7A11 was determined by qRT-PCR. B Cell viability, C MDA, D GSHand E iron levels were assessed *, P < 0.05, **, P < 0.01, ***, P < 0.001