Skip to main content
Fig. 7 | Journal of Biomedical Science

Fig. 7

From: Reciprocal deregulation of NKX3.1 and AURKA axis in castration-resistant prostate cancer and NEPC models

Fig. 7

AURKA negatively regulates NKX3.1 in NEPC. A AURKA overexpression in 49F cells leads to lowering of NKX3.1 expression levels. B Quantitative analysis of AURKA, NKX3.1, synaptophysin, enolase and tubulin levels upon ectopic overexpression of AURKA. The data, obtained from three independent experiments, were normalized to tubulin. *P < 0.05, **P < 0.01. C AURKA knockdown enhances NKX3.1 protein levels in 49F cells. D Histogram representing the quantitative analysis of detected protein levels from three independent experiments. *P < 0.05, **P < 0.01. E 3A-NKX3.1 shows higher expression as compared to NKX3.1 in 49F cells. F Quantification of NKX3.1, AURKA, synaptophysin and enolase levels normalized to tubulin from three independent experiments. *P < 0.05, **P < 0.01. G 3A-NKX3.1 is most poorly ubiquitylated as compared to control 49F and WT-NKX3.1 overexpressing 49F cells. H Micrographs showing changes in neurite outgrowth upon ectopic overexpression of NKX3.1 and 3A-NKX3.1 in 49F cells. I Histogram representing neurite length (number of cell body widths). Quantification was performed by observing ten different fields of cells, in five different replicates. J Histogram showing effect of WT and 3A-NKX3.1 overexpression on viability of 49F cells. K Schematic model describing the plausible pathway of AURKA-NKX3.1 signaling in CRPC and NEPC pathogenesis

Back to article page