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Fig. 7 | Journal of Biomedical Science

Fig. 7

From: Modular self-assembly system for development of oligomeric, highly internalizing and potent cytotoxic conjugates targeting fibroblast growth factor receptors

Fig. 7

Superior cytotoxicity of the tetrameric FGF1-SA conjugate against FGFR1 producing cells. A–C Cytotoxic potential of MMAE-FGF1E-AviTag-Biot and MMAE-FGF1E-AviTag-Biot-SA-4A was measured in various cell lines: U2OS (A), U2OS-R1 (B) and DMS114 (C). D and E Control cytotoxicity of non-conjugated FGF1E-AviTag-Biot and FGF1E-AviTag-Biot-SA-4A was measured in U2OS (D) and U2OS-R1 (E) cells. All cells were treated with the indicated agents at various concentrations for 96 h and their viability was assessed with the Presto Blue assay (AE). Results are mean values from three independent experiments ± SD. Statistical significance: *p < 0.05, **p < 0.01, ***p < 0.0001, n.s.- not significant. F EC50 values of analyzed proteins were calculated based on the Hill equation using Origin 7 software (Northampton, MA). 4 ×—monomeric non-conjugated FGF1E-AviTag-Biot and monomeric conjugated MMAE-FGF1E-AviTag-Biot were used in fourfold higher concentrations in the experiments to provide cells with equal molar concentrations of drug and targeting molecule

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