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Fig. 5 | Journal of Biomedical Science

Fig. 5

From: Enhanced enzymatic production of cholesteryl 6ʹ-acylglucoside impairs lysosomal degradation for the intracellular survival of Helicobacter pylori

Fig. 5

Subcellular fractionation analysis of H. pylori-infected AGS cells. AGS cells were infected with H. pylori 26695 for 6 h and lysed to generate total lysates (TL) that were subjected to subcellular fractionation using the Minute™ Plasma Membrane Extraction and Subcellular Fractionation kit. The following fractions were analyzed: nucleus (NU), cytosol (CY), organelles (OR), and plasma membrane (PM). a After protein quantification, TL and all fractions were examined by immunoblotting with the corresponding antibodies: anti-histone H3 (nucleus), anti-GAPDH (cytosol), anti-Rab7 and anti-LC3B (specific to late endosomes and autophagosomes, respectively), and anti-Na+K+ ATPase (plasma membrane). b CGAT activity of the fractions. c The distribution of CGAT activity in the NU, CY, OR, and PM. Total CGAT activity in each fraction was calculated by multiplying the specific activity (shown in b) by the amount of protein in each fraction. The plot shows the percentage of CGAT activity in each fraction. The data represent the mean ± SEM. **p < 0.01, *p < 0.05 vs. the control (n = 3)

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