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Fig. 6 | Journal of Biomedical Science

Fig. 6

From: Enhanced enzymatic production of cholesteryl 6ʹ-acylglucoside impairs lysosomal degradation for the intracellular survival of Helicobacter pylori

Fig. 6

Lack of CAG increases lysosomal enzyme activity and causes accumulation of lysosomes. a AGS cells were pretreated with CAG, infected with H. pylori 26695 or ΔCGT as described in Fig. 3b, and then stained with LysoTracker™ Red DND-99 (red). Scale bar: 10 μm. b More than 100 cells from each group of (a) were counted. c Samples were prepared as described in Fig. 3b, followed by staining with cathepsin B substrate Magic Red™ (red). Scale bar: 10 μm. d More than 100 cells from each group in (c) were counted. e AGS cells treated with CAG, infected with H. pylori 26695 or ΔCGT as described in Fig. 3b were immunostained for Lamp-1 (green). f More than 100 cells from each group were counted. g GES-1 cells were treated with CAG, infected with H. pylori 26695 or ΔCGT as described in Fig. 3b, and then immunostained for Lamp-1 (green). h More than 50 cells from each group were counted. In panels (b, d, f, and h), the data represent the mean ± SEM. **p < 0.01, *p < 0.05 vs. the control (n ≥ 50). i Subcellular fractions of H. pylori 26695- and ΔCGT-infected AGS cells were analyzed for the levels of Lamp-1, cathepsin D, and β-actin. Thirty microgram of protein from each fraction were loaded for the analysis, according to the BCA method of protein quantification

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