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Fig. 1 | Journal of Biomedical Science

Fig. 1

From: Transforming growth factor-β1 decreases erythropoietin production through repressing hypoxia-inducible factor 2α in erythropoietin-producing cells

Fig. 1

C3H10T1/2 cells exhibit a fibroblastic phenotype. a Bright-field images of C3H10T1/2 (referred to as 10T1/2) cells on cell culture dishes at the indicated time points after culture. Original magnification, ×400. Scale bar: 200 µm. bf The relative mRNA expression normalized by Hprt. Cdh1 encodes the epithelial cell marker E-cadherin; Vegfr2 encodes the endothelial cell marker vascular endothelial growth factor (VEGF) receptor 2; Nphs1 and Nphs2 encode the podocyte markers nephrin and podocin, respectively; Pdgfra, Pdgfrb, Acta2, Ng2 and Nt5e encode the fibroblast markers, platelet-derived growth factor receptor (PDGFR) α, PDGFRβ, α-smooth muscle actin (α-SMA), neuron-glial antigen 2 and ecto-5′-nucleotidase, respectively; Ki67 encodes the marker for the assessment of cellular proliferative activity, Ki67 antigen; Hprt encodes hypoxanthine–guanine phosphoribosyl transferase and serves as the internal control. The gene expression of 10T1/2 cells is compared with those of 3T3 cells and the kidney from C57BL/6 wild type (WT) mice. The average expression of the kidney is set at 1. ND, not detected. The data are expressed as the mean ± standard error of the mean (SEM). n = 4 per group. *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA with Tukey’s test

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