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Fig. 2 | Journal of Biomedical Science

Fig. 2

From: Transforming growth factor-β1 decreases erythropoietin production through repressing hypoxia-inducible factor 2α in erythropoietin-producing cells

Fig. 2

10T1/2 cells produce erythropoietin. a The relative mRNA expression normalized by Hprt. Epo, Vegfa, Hif1a, Epas1, Egln1, Egln3 and Slc2a1 encode erythropoietin (EPO), VEGF-A, hypoxia-inducible factor 1α (HIF1α), HIF2α, prolyl hydroxylase domain (PHD) 2, PHD3 and solute carrier family 2 member 1, respectively. 10T1/2 cells and 3T3 cells are analyzed after 24-h exposure to normoxia (N, 21% O2) or hypoxia (H, 0.5% O2). The mRNA expression of each gene in cells are compared to that of the kidney from the control mice (Cont) and those one day after phlebotomy (Phl). The average gene expression level of kidney from Cont mice is set at 1. n = 4 per group. ND, not detected. b EPO concentration in the supernatant of 10T1/2 cells after 24-h exposure to normoxia (N) or hypoxia (H). n = 4 per group. c The relative Epo expression normalized by Hprt in 10T1/2 cells after 24-h exposure to different concentrations of PHD inhibitor (PHDi, roxadustat) (left panel) or after exposure to PHDi (roxadustat 50 µM) for the indicated durations (right panel). n = 4 per group. Data are expressed as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA with Tukey’s test for cell experiments in a and c, Student’s t test for animal experiments in a and cell experiments in b

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