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Fig. 3 | Journal of Biomedical Science

Fig. 3

From: Transforming growth factor-β1 decreases erythropoietin production through repressing hypoxia-inducible factor 2α in erythropoietin-producing cells

Fig. 3

Hypoxia induces Epo through HIF2α in 10T1/2 cells. a Representative Western blot analysis for the expression of HIF1α and HIF2α in hypoxia chamber (0.5% O2) for the indicated duration. Right panels showing the expression of HIF1α and HIF2α normalized by β-actin. n = 3 per group. b Schema illustrating the siRNA transfection specific for Hif1a (siHif1a), Epas1 (siEpas1) or control (siControl) and culture medium refresh. HIFs are analyzed after 6-h exposure to normoxia or hypoxia. c Upper panel showing the representative Western blot analysis for the expression of HIF1α and HIF2α after siRNA transfection and exposure to normoxia/hypoxia as indicated. Lower panels showing the expression of HIF1α and HIF2α normalized by α-tubulin. n = 3 per group. d Schema illustrating the siRNA transfection and culture medium refresh. Gene expression is analyzed after 24-h exposure to normoxia or hypoxia. d The relative mRNA expression of Epo, Hif1a and Epas1 normalized by Hprt. n = 3 per group. Data are expressed as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA with Tukey’s test in a, c and e

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