Fig. 7

TGF-β1 inhibits HIF2α and Epo expression through ALK5 in 10T1/2 cells. a The relative Epas1 expression normalized by Hprt in cells after exposure to TGF-β1 for the indicated duration. b Schema illustrating the analysis of HIFs in cells after 6-h exposure to normoxia or hypoxia in the presence or absence of TGF-β1. c Representative Western blot analysis for HIF1α, HIF2α and β-actin in cells of the experiment in b. Lower panels showing the expression of HIF1α and HIF2α which is normalized by β-actin. n = 3 per group. d Schema illustrating the analysis of HIFs in cells after 6-h exposure to normoxia or hypoxia in the presence or absence of TGF-β1 with or without ALK5i SB431542. e Representative Western blot analysis for HIF1α, HIF2α and α-tubulin in cells of the experiment in d. Lower panels showing the expression of HIF1α and HIF2α which is normalized by α-tubulin. n = 3 per group. f Schema illustrating the analysis of gene expression in cells after 24-h exposure to normoxia or hypoxia in the presence or absence of TGF-β1 with or without ALK5i SB431542. g The relative mRNA expression of Epo, Hif1a and Epas1 normalized by Hprt in cells of the experiment in f. n = 4 per group. Data are expressed as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA with Tukey’s test