Table 2 Targeting of KRAS related molecules in cancer via CRISPR systems (information is provided first based on cancer type, then in chronological order.)

CRISPR/Cas9

Lung

MUC1-C

A549/KRAS(G12S), H460/KRAS(Q61H), H358/KRAS(G12C), H441/KRAS(G12V), H2009/KRAS(G12A), H1975/EGFR(L858R/T790M), PC9GR/EGFR(delE746_A750), H838s(mutant p53) and HEK293T

NCR nu/nu

2016

[127]

CRISPR/Cas9

Lung

KEAP-1

A549, H2030, H2009, KP1, KP2 and KPK

Kras^LSL−G12D/+; p53^flox/flox (KP)

2017

[128]

CRISPR/Cas9

Lung

KEAP-1

NCI-H1299, HCC364, NCI-H1975, and HCC827

Nude mice

HCC827 KEAP1^KO

2017

[131]

CRISPR/Cas9

Lung

Smarca4 (Brg1), Arid1a and Setd2

HEK293 FT

C567B6/129Sv4: Kras^LSL−G12D and Kras^LSL−G12D;p53^flox/flox

2017

[132]

CRISPR/Cas9

Lung

MEK-ERK-PI3K pathways

HCT116, SW620, CRC119 and CRC240

NOD/SCID gamma mice or nude mice

2017

[133]

CRISPR/Cas9

Lung

PI3K pathway

HCT116, DLD1, CT26 and AALE

BALB/c mice

2017

[134]

CRISPR/Cas9

Lung

Utx

Kras^G12D/+ MEFs, KP cells, KPU cells, and HEK-293 T

Kras^G12D/+, Trp53^L/L, Lkb1^L/L, and Utx^L/L mice

2018

[135]

CRISPR/Cas9

Lung

MAPK7

MOR (ECACC), NCI-H2122 (ATCC), A549 (ATCC), NCI-H441 (ATCC)

NCr nu/nu mice

2018

[143]

CRISPR/Cas9

Lung

NF1

KP.1, KP.2, LKR10 and LKR13 mouse

KRAS‐mutant/TP53‐WT/NF1‐WT (PDK), KRAS‐mutant/TP53‐WT/NF1‐mutant (PDKN1 and PDKN2) human

LSL‐Kras ^G12D; Trp53 ^flox (KP) mice C57BL/6–129/Sv

2019

[138]

CRISPR/Cas9

Lung

STK11/LKB1 and SIK1, SIK3

A549, 634 T KP, VB-mouse lung tumor-derived KP and KPSik1 cells

Kras (KrasLSLG12D/ + ; R26LSL;luc/luc);

KL (KrasLSLG12D/ + ; Lkb1fl/fl;R26LSL;luc/luc)

KP (KrasLSLG12D/ + ; p53fl/fl;R26LSL;luc/luc)

KPL (KrasLSLG12D/ + ; Lkb1fl/fl;p53fl/fl;R26LSL;luc/luc);

Sik1^fl/fl and Sik2^fl/fl mice

2019

[139]

CRISPR/Cas9

Lung

STK11/LKB1 and SIK family

A549, H2122, H2126, H1650

Kras^LSL−G12D, p53^flox, Lkb1^flox, H11^LSL−Cas9 and Rosa26^{LSL−tdTomato} mice

2019

[172]

CRISPR/Cas9

Lung

PCDH7

HBECs, H1944

PCDH7 transgenic mice

C57BL/6 J strain

Kras^LSL−G12D; PCDH7^LSL/LSL; Kras^LSL−G12D; Tp53^fl/fl

2019

[141]

CRISPR/Cas9

Lung

Pancreas

SHOC2

CFPAC-1, A549, NCI-H23, KP4, MIA PaCa-2, NCI-H2030, Panc 10.05, HCC364, NCI-H1299, HCT116, LOVO, PA-TU-8902, NCI-H1975, NCI-H2009, SU.86.86, MDA-MB-157, and MDA-MB-436

SCID Hairless Outbred (SHO) mice

2019

[142]

CRISPR/Cas9

Lung

EGFR

A549

n/a

2020

[144]

CRISPR/Cas9

Lung

STK11 and PTEN

n/a

n/a

2021

[151]

CRISPR/Cas9

CRC

Apc and Trp53

Lgr5 + stem cells murine AKP and human LS174

Apc^fl/fl, Kras^LSL−G12D/+, Villin^CreER,, Lgr5^eGFP−CreE, Lgr5^CreER, Rosa26^{LSL−Cas9−eGFP} and Rosa26^{LSL−tdTomato}

2017

[145]

CRISPR/Cas9

CRC

ERN1-JNK-JUN

HEK293

n/a

2018

[146]

CRISPR/Cas9

CRC

TGFBR2

HepG2 and HEK293T

NOD scid gamma (NSG) mice

2020

[147]

CRISPR/Cas9

Pancreas

PI3K

PACO9 and PACO19

LSL-Kras ^G12D; p53 ^flox/flox; Pdx1-CreER, LSL-Kras ^G12D; p53 ^R172H/WT; Pdx1-Cre (KPC) and A13 clones

2017

[110]

CRISPR/Cas9

Pancreas

GALNT3 and B3GNT3

SW1990, Capan1, NSP and SP

Kras^G12D; Pdx-1-Cre (KC) and Kras^G12D; p53^R172H; Pdx-1-Cre (KPC)

2018

[148]

CRISPR/Cas9

Pancreas

ISG15

Panc02

C57BL/6

2019

[149]

CRISPR/Cas9

Pancreas

PRKD1

Panc-1, WI-38

PRKD1^KO, KC, PRKD1^KO−KC, p48Cre, LSL-KrasG12D and NSG

2020

[150]