Fig. 7

Mass spectrometry proteomics of differentially expressed proteins from Ctrl and KO MEFs. A, B Pie charts of the expression profiles (KO/Ctrl) (left panel) and subcellular distributions (right panel) of identified proteins from cells without IR treatment (Ctrl 0 and KO 0) (A) and 4 h post-IR exposure (Ctrl 4 and KO 4) (B). Up-regulated, KO/Ctrl fold-change ≥ 1.5 (p < 0.05); down-regulated, fold-differences ≤ 0.67 (p < 0.05). C, D Ingenuity Pathway Analysis (IPA) showing ten of the top most interesting canonical pathways significantly modulated by differentially expressed proteins in KO cells compared to Ctrl cells (p < 0.05) without IR treatment (C) and 4 h post-IR exposure (D). The bar represents − log (p-value) for each pathway. E Differentially expressed proteins identified in the canonical pathway of “role of BRCA1 in DNA damage response” from the two sets of cells (irradiated and non-irradiated). F, G IPA showing the top six diseases and biological functions identified for differentially expressed proteins in KO cells compared to Ctrl cells without IR treatment (F) and 4 h after IR exposure (G). H IPA showing the top upstream regulators from the two sets of cells (irradiated and non-irradiated). I Venn diagram showing the number of proteins identified from the two sets of cells (irradiated and non-irradiated) by LC–MS/MS, as well as the number of shared proteins. J IPA showing the top six diseases and biological functions of overlapping proteins identified between the two sets of cells (irradiated and non-irradiated)