Fig. 1

ROR2 inhibits the proliferation of melanoma cells. A Expression of ROR2 in human melanoma cell lines. ROR2 levels were normalized to GAPDH and expressed as fold change (FC) relative to UACC903 cells. B, C ROR2 inhibits proliferation of melanoma cells. Crystal violet assays were performed in A375 cells stably transduced with either control (Empty) or a ROR2-expressing plasmid (B) and in MeWo cells stably transduced with either control (scramble) plasmid or two shRNA for ROR2 (C4 and C2) (C). Statistical significance was tested by a one-tailed Student’s T-Test (B) or ANOVA (C). ROR2 overexpression and silencing was determined by western blot. D ROR2 expression alters the doubling time of melanoma cells. E, F ROR2 impairs cell-cycle progression. Flow cytometry analysis of PI-stained A375 cells overexpressing ROR2 (E) and MeWo cells upon silencing of ROR2 (F). Bar graph shows the mean ± S.D. of the percentage of cells in each phase of the cell-cycle. Statistical significance was tested by a one-tailed Student’s T-Test (E) or ANOVA (F). *: p < 0.01, **: p < 0.001, ***: p < 0.0001, n.s.: not significant