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Table 3 Effect of drugs on [3H]VPA uptake in WT and MT ALS cell lines

From: Monocarboxylate transporter functions and neuroprotective effects of valproic acid in experimental models of amyotrophic lateral sclerosis

Compounds

Concentration

[3H]VPA uptake (% of control)

(mM)

WT(hSOD1WT)

MT (hSOD1G93A)

Control

 

100 ± 7

100 ± 3

+ Ibuprofen

1

64.3 ± 3.6**

61.9 ± 7.5**

 

10

22.3 ± 1.5***

33.5 ± 1.0***

+ Edaravone

1

80.3 ± 6.0*

81.0 ± 5.4*

 

10

67.3 ± 7.6**

68.6 ± 6.3**

+ Furosemide

1

61.7 ± 6.3***

61.7 ± 2.2***

 

5

77.5 ± 4.9**

82.3 ± 7.5 **

+ DIDS

1

54.8 ± 4.3***

58.8 ± 4.9***

+ Estrone − 3 sulfate

10

60.6 ± 8.4***

58.2 ± 8.5***

  1. [3H]VPA uptake was analyzed for 10 s at 37 °C under acidic pH in the presence of drugs at concentrations of 1–10 mM. The data represent mean ± SEM (n = 3–4). ***p < 0.001, **p < 0.01, and *p < 0.05 represent significant difference from the respective WT and MT controls. DIDS, 4,4′-diisothiocyano-2,2′-stilbenedisulfonic acid
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Journal of Biomedical Science

ISSN: 1423-0127