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Fig. 5 | Journal of Biomedical Science

Fig. 5

From: SLCO4A1-AS1 promotes colorectal tumourigenesis by regulating Cdk2/c-Myc signalling

Fig. 5

SLCO4A1-AS1 acts as a scaffold to promote the association between Cdk2 and Hsp90. a Protein expression of Cdk2 and Hsp90 in the SLCO4A1-AS1 overexpressing or SLCO4A1-AS1-depleted CRC cells. b Co-immunoprecipitation (Co-IP) assays were performed to detect the interaction between Hsp90 and Cdk2. c SLCO4A1-AS1 knockdown impairs the interaction between Hsp90 and Cdk2 in CRC cells. d Immunoblot analysis of Cdk2 in SW620 cells transfected with shCtrl and shSLC plasmids. Cells were treated with NH4Cl (25 mM) or MG132 (20 μM) for 3 h before harvest. e SLCO4A1-AS1 knockdown decreased the protein stability of Cdk2. Cells were treated with CHX (50 μg/ml) as indicated before harvest. f Hsp90 blocked SCLO4A1-AS1 knockdown-induced ubiquitination modification of Cdk2. 293 T cells were co-transfected with different plasmids as indicated, and their lysates were subjected to protein ubiquitination assays using Myc-Tag beads. *P < 0.05; **P < 0.01

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