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Fig. 3 | Journal of Biomedical Science

Fig. 3

From: LRG1: an emerging player in disease pathogenesis

Fig. 3

LRG1 expression in normal and cancer tissues. A A polyclonal (Proteintech) or monoclonal (Magacizumab) antibody was used for the detection of human LRG1 (brown) respectively in human liver (left, scale bar: 250 µm) and testis (right, scale bar: 100 µm). B Immunohistochemistry showing upregulation of LRG1 expression (brown) in human breast cancer (right) compared to healthy control (scale bar: 60–62 µm). The arrow indicates an example of LRG1pos blood vessel. C Lrg1 mRNA (green) detected by RNA scope and immunofluorescence for Collagen IV (white) profiling tissue vessels in normal mouse liver (scale bar: 100 µm). D Lrg1 mRNA (red) detected by RNA scope and immunofluorescence for Collagen IV (white) profiling tissue vessels in normal mouse lung (scale bar: 100 µm). The arrow points to Lrg1 mRNA expressed by lung alveolar epithelium while the box shows details of additional LRG1pos stromal cells. E An anti-human polyclonal antibody (Proteintech) was used for the detection of LRG1 (red) in normal human lung (scale bar: 100 µm). F Upregulation of LRG1 expression in murine metastatic lung tumours. Top: examples of Lrg1 mRNA detected by RNA scope (green) and Collagen IV stained by immunofluorescence (white); left: low magnification of metastatic tumour mass (scale bar: 100 µm); middle: Lrg1 expression by cancer cells or cancer-associated fibroblasts (scale bar: 50 µm); right: Lrg1 expression by tumour vessels (scale bar: 50 µm). Bottom left: Lrg1 mRNA (green) detected by RNA scope and immunofluorescence for Podoplanin (red) (scale bar: 100 µm); right: Lrg1 mRNA detected by RNA scope (green) and immunofluorescence for the endothelial markers ERG (red) and Podocalyxin (white) (scale bar: 50 µm). Murine metastatic tumour samples were kindly provided by M. Singhal and H. G. Augustine, Heidelberg University, Germany. Human samples were purchased from Biomax and Covance.

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