Fig. 2

Binding of Met to CAV-1 and endocytosis of Met in HTR8/SVneo cells exposed to hypoxia. A–B Results of real-time quantitative PCR showing the expression of Met and CAV-1 in HTR8/SVneo cells that were exposed to various concentrations of O₂ for 1 h to 12 h. C–D Typical results (C) and statistical results (D) of Co-IP showing the interaction of Met and CAV-1 in HTR8/SVneo cells that were exposed to 20% or 2% O₂ for 6 h to 48 h. E Schematic diagram illustrating the procedure for the biotinylation internalization assay. Details are described in the Methods, and the small black dots indicate the biotin-labeled cell-surface proteins. F–G Typical results (F) and statistical results (G) of the biotinylation internalization assay of Met in HTR8/SVneo cells that were transfected with or without a specific siRNA for CAV-1 and exposed to 20% or 2% O₂ for 4 h. All statistical analyses were performed based on the results of three independent experiments. The data are presented as the mean ± SEM, and comparisons between groups were analyzed by one-way ANOVA followed by the Tukey–Kramer multiple comparisons post hoc test. *Comparison between the indicated groups, p < 0.05