Fig. 3
Colocalization of Met with the proteasome and the expression of Cbl in HTR8/SVneo cells under hypoxia. A–B Typical results of the high-resolution immunofluorescence analysis of Met (a–e) and Met/LMP2 (f–j) in HTR8/SVneo cells that were exposed to 20% or 2% O2 for 0 h to 48 h. Scale bar, 20 μm. C Quantitative statistics of Met punctate aggregation at the indicated times (A-B). Line chart showing the numbers of Met punctate aggregates per μm2 in HTR8/SVneo cells. D Line chart showing the ratio of Met-LMP2 colocalization to Met puncta in the cells, as determined by the statistical analysis of three sections per group and 5 random fields of view in each section. E Results of real-time quantitative PCR showing the expression of Cbl in HTR8/SVneo cells that were exposed to various concentrations of O2 for 1 h to 48 h. The data are expressed as the mean ± SEM and were analyzed by the two-tailed t test based on at least three independent experiments. *Compared with the corresponding data in the control, p < 0.05