Fig. 4
The distribution of Met aggregates and proteasomes in HTR8/SVneo cells upon Cbl knockdown. A Results of real-time quantitative PCR showing the expression of Cbl in HTR8/SVneo cells transiently transfected with a specific siRNA for Cbl (si-Cbl) or a scramble siRNA (NC) or exposed to only the transfection reagent (mock). B Typical results of the high-resolution immunofluorescence analysis of Met (a–d), LMP2 (e–h), and Met/LMP2 (i–l) in HTR8/SVneo cells transfected with NC, si-Cbl for 48 h or exposed to the proteasome inhibitor MG132 (5 μM). Scale bar, 20 μm. C Bar chart showing the numbers of Met punctate aggregates per μm2 in HTR8/SVneo cells. D Bar chart showing the ratio of Met-LMP2 colocalization to Met puncta in the cells, as determined by the statistical analysis of three sections per group and 5 random fields of view in each section. The data are presented as the mean ± SEM, and comparisons between groups and were analyzed by one-way ANOVA (for normally distributed data) followed by the Tukey–Kramer multiple comparisons post hoc test. *Compared with the corresponding data in the control, p < 0.05