Fig. 2
Inhibition of viral growth by scFv07 in cells. A RD cells were infected with different titers (0, 1, and 10 TCID50) of EV-A71 at 2 days post-transfection with scFv07 expressing plasmid (pTT5-scFv07) or pTT5 vector control. After 24 h of infection, the cells were fixed, and ELISA was performed by using anti-EV-A71 antibody to determine the expression of viral antigen. The absorbance of 450 nm was determined. B Post-transfection with scFv07 expressing pTT5-scFv07 plasmid or pTT5 vector cells were infected with EV-A71 (M.O.I. = 0.1) for 24 h followed by staining with anti-HA tag and EV-A71 antibody (MAB979). Magnification: 10x. C Viral supernatants from above EV-A71-infected cells as indicated were used to calculate virus titer by plaque assay. D Viral growth (M.O.I. = 0.01) of pTT5 or pTT5-scFv07 expressed RD cell were examined at 6, 12, 24 and 36 h post infection by plaque assay. *p < 0.05; **p < 0.001