Fig. 3
Intracellular scFv07 and 07-IgG1 do not recognize EV-A71 capsid proteins. A Cells were infected with EV-A71 (MOI 0.1) for 24 h followed by staining with rabbit anti-HA tag and mouse anti-EV-A71 (Mab979) antibody. Goat anti-mouse IgG-488 and goat anti-rabbit IgG-594 were used for further detection. B EV-A71 infected cell lysate (500 μg) was used for immunoprecipitation assays. MAB979 (2 μg) was captured by protein G magnetic beads (20 μL) and incubated with lysate for 24 h at 4 °C. After several washes, eluents were analyzed by WB using MAB979 and anti-HA tag antibody. C PEG precipitated EV-A71 were coated onto ELISA wells. Serial dilution of 07-IgG1 and MAB979 were added as primary antibody and incubated at 37 °C for 1 h. HRP labeled goat anti-mouse and human IgG were used for detecting bound antibody