Fig. 5

ENO1 is upregulated in EV-A71 infection and enhances viral replication. A ENO1 protein expression of EV-A71 infected RD cells were examined by WB. The fold change of ENO1 was normalized with the intensity of α-tubulin. B RD cells were infected EV-A71 (M.O.I. = 0.01) and ENO1 expression was analyzed at different hours post infection (h.p.i.). C ENO1 overexpressed RD cells were infected with EV-A71 for 24 h at the indicated M.O.I. of 0.001 and 0.01. Virus titers in the supernatant were determined by plaque assay. D ENO1 knock-down or supplement with ENO1 RD cells were infected with EV-A71, and viral titers were determined by plaque assay. E ENO1 overexpressed or knock-down RD cells were infected with EV-A71 at M.O.I. of 0.01 and virus growth was analyzed by plaque assay. *p < 0.05; **p < 0.001, ***p < 0.0001