Fig. 2

CDK9 inhibition increases O-GlcNAcylation of MRE11. A HCFC1 was used as a positive control to confirm the efficiency of immunoprecipitation. LNCaP and LN95 cells were treated with CDK9 inhibitor for 4 h and the O-GlcNAcylated proteins were enriched using RL2 and CTD110.6 antibodies, followed by mass spectrometry analysis. The data presented are average of three biological replicates (signal intensity). B Scatter plots showing the O-GlcNAcylated proteins. The signal intensity values represented here were normalized to IgG. C Venn diagrams showing the proteins that had > 20% increased O-GlcNAcylation as compared to DMSO in all three biological replicates: we considered these as the hyper-O-GlcNAcylated proteins. D Bar plots indicating the O-GlcNAcylation intensity of MRE11 in LNCaP and LN95 cells. The values are average of three biological replicates. First, values were normalized to negative immunoprecipitation, and second, data are presented relative to control (DMSO), which was set to value of one