Fig. 3
The suppressive effects of the combination treatment using docetaxel and CAPE on PC/DX25 xenografts in nude mice. A Docetaxel-resistant PC/DX25 cells were injected subcutaneously on both flanks (1 × 106 cells/side) of the nude mice. Tumors were allowed to grow for 2 weeks to reach an average volume larger than 100 mm3. Mice were randomly separated into control group (receiving 5 mg/kg docetaxel twice per week via i.p. injection) or combination treatment group (receiving 5 mg/kg docetaxel plus 10 mg/kg CAPE, both twice per week via i.p. injection). Tumor growth was monitored for 29 days before mice were sacrificed. Tumors were measured twice per week with absolute digimatic calipers. Tumor volume was calculated with the formula volume = length x width x height × 0.52. B Body weight of mice in control group and combination treatment group was measured at the same time as tumor volume measurement. The body weight in each group was shown as mean ± standard error. C Photographs of xenografts in control group (docetaxel treatment only) and in combination treatment group (docetaxel plus CAPE). D Photographs of mice being dissected were shown for images of blood vessels nearby tumors. All data were percentage of that. Results present as mean ± standard error (n ≥ 3; **p < 0.001; ***p < 0.0001 v.s. control)