Fig. 6
Effect of C3A on hair growth cycles in an AGA mice model. A Animal experimental schedule for inducing androgenetic alopecia mice model. DHT (1 mg/100 μl) was intraperitoneally injected and C3A (500 μM), SB203580 (1 mM), and Apocynin (100 mM) was topically administered once every 2 days. B Photographs were taken on days 7, 11, 15 and 19 after depilation. C Depilated mice skin tissue was frozen sectioned and histologic examination was performed by HE staining. Images were captured by digital slide scanner. N = 6. Scale bars are 400 μm. D Hair follicle number was counted in the same random area (1 mm2). E Hair bulb diameter was measured. F Skin thickness was assessed. G Dermal ratio was rated by measuring epidermis and dermis length of skin tissue section. H Immunofluorescence staining of Ki67 in the dorsal skin was performed with anti-Ki67 antibody and DAPI for nuclei staining. Magnification × 200. Scale bars are 40 μm. *p < 0.05 versus Control. #p < 0.05 versus DHT + Vehicle