Fig. 3

Ribosome profiling process where ribosome footprints are obtained for deep sequencing. Isolation of ribosome-bound mRNAs is conducted through treatment of non-specific nucleases such as RNase I or micrococcal nuclease). Ribosome footprints (showing positioning between start and stop codon of gene) are then used for library generation and deep sequencing. Identification of novel small peptides made possible by isolation of actively translated regions of the transcript, which is directly mapped back to genomic coding regions