Fig. 4

Effect of PGE2 on mitochondrial function. a RNA expression in cell line from patient with glioblastoma and paired glioblastoma specimens. b Mitochondrial morphology was fluorescently labelled by the pDsRed 2-Mito expression clone. Statistical data were analysed by performing two-tailed unpaired Student’s t test. Scale bar is 50 μm. c Effect of TMZ on mitochondrial fusion-related protein expression. d Mitochondrial activities of U87MG, U87MG-R, and U87MG-Sp1KO cells determined by performing Seahorse XF Mito Stress tests. e Mitochondrial activity determined via Seahorse XF Mito Stress tests after treatment with PGE2 for four days. f Mitochondrial activity after treatment with TMZ in the presence or absence of PGE2 for four days. g Mitochondrial activity after treatment with PGE2 in the presence of each antagonist for four days. Statistical data were analysed by performing two-tailed unpaired Student’s t test