Fig. 1

STIL is up-regulated in lung cancer patients and correlated with poor prognosis. a STIL expression determined by microarray analysis of 9 normal lung cell lines and 166 lung cancer cell lines derived from GEO. b STIL expression was determined by RNA sequencing of 100 solid tissue normal samples (referred to non-malignant lung tissues) and 740 ADCs and SCCs derived from TCGA, and the score for the AUC are shown (right panel). c STIL protein levels were analyzed by IHC analysis of 41 normal lung tissue samples, 280 primary lung cancers, and 115 paired metastatic lymph nodes (derived from the pool of 280 primary lung cancers). The normal lung tissues were used as non-malignant comparators for these lung cancers and metastatic lymph nodes. Representative IHC images of STIL staining are shown (left panel). Scale bar: 20 μm. IHC staining intensity was grouped as 0 (negative), + (weak), ++ (moderate), and +++ (strong), and the percentage distribution is depicted (right panel). d STIL expression was determined by microarray analysis of 955 lung ADCs and 28 brain metastases derived from lung ADCs. These data were derived from GEO. e Kaplan–Meier survival analysis of STIL gene expression was performed using microarray data of 1926 lung cancers derived from Kaplan–Meier Plotter. The median survival of the two groups is shown. Significance is determined by the log-rank test (p < 0.0001). The histologic subtypes of lung cancer in b–e are described in Additional file 1: Table S2. Data information: In a, b and d, the red lines indicate the median. In a–d, significance is determined by t-test (***p < 0.001)