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Fig. 1 | Journal of Biomedical Science

Fig. 1

From: Unravelling the physiological roles of mazEF toxin–antitoxin system on clinical MRSA strain by CRISPR RNA-guided cytidine deaminase

Fig. 1

Mutation of mazEF TA gene and its effect on growth rate in MRSA P-1780 strain. a Sequencing chromatogram revealed successful point mutation of C to T (marked in red) converting Q14 of mazE and Q16 of mazF, into a premature stop codon respectively. b Reduction in CFU count/ml post 16 h growth, after the transduction of the pnCasSA-BEC-mazEsp plasmid generating mazE mutants compared to mazF mutants. c Growth curve analysis of the wildtype, mazE and mazF mutants grown at 37 °C for 8 h in the TSB broth, for analyzing any growth defects

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Journal of Biomedical Science

ISSN: 1423-0127