Fig. 5

Myricetin impairs tumor growth in C4-2B xenografts. A Comparison of cell viability of C4-2B treated with free-form myricetin (Free M) or PLGA-encapsulated myricetin (PLGA-M). Cells were treated with different concentrations of M or PLGA-M for 24 h, followed by MTT assay. B C4-2B cells (1 × 10⁶) were implanted subcutaneously in the hindlimb of nude mice. PLGA-myricetin formulation (PLGA-M) (intraperitoneal injected, 20 mg/Kg, three times a week), enzalutamide (Enza) (oral gavage feeding, 12.5 mg/Kg, five times a week), and the combined treatment (Enza + PLGA-M) were administered respectively after 14 days implantation. Tumor volumes were measured for up to 3 weeks. Tumor volume was calculated using the formula (length × width × height × 0.52). Statistical significance was evaluated using Student’s t-test. C Representative IHC images of Ki67 and CD31 analysis of xenograft tumor sections from mice treated with vehicle, PLGA-M, Enza, or Enza + PLGA-M. D The proportion of Ki67 + cell was rescored in each field (400 ×) for six random fields in each group of IHC images. CD31 positive vessels in each field (100 ×) were scored for six random fields in each group. Significance was calculated using Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001