Skip to main content
Fig. 4 | Journal of Biomedical Science

Fig. 4

From: Inhibition of ChREBP ubiquitination via the ROS/Akt-dependent downregulation of Smurf2 contributes to lysophosphatidic acid-induced fibrosis in renal mesangial cells

Fig. 4

LPA induces the production of ROS, and ROS scavenging decreases the LPA- or ROS-induced expression of ChREBP and fibrotic factors in SV40 MES13 cells. A SV40 MES13 cells were treated with 10 µM LPA for the indicated durations. B SV40 MES13 cells were treated with LPA in the presence or absence of ki16425 for 1 h. A and B The intracellular levels of ROS were measured by flow cytometry with DCFH-DA. The values in the representative flow cytometry histograms indicate the fluorescence intensity of DCF in the whole cells. The relative fluorescence intensity levels of DCF were compared with the production of ROS in the control cells. C The cells were pretreated with NAC for 1 h and subsequently treated with LPA for 3 h. D The cells were pretreated with NAC for 1 h and subsequently treated with H2O2 for 1 h. C and D The protein levels of ChREBP, fibronectin, TGF-β, and IL-1β were analyzed by western blotting, quantified using ImageJ software, and normalized to those of β-actin. The data are presented as the mean ± SEM of results obtained from three independent experiments. *p < 0.05, **p < 0.01 vs. Con; #p < 0.05, ##p < 0.01 vs. LPA or H2O2

Back to article page