Fig. 7

Inhibition of Akt signaling suppresses the alterations in the expression of ChREBP and Smurf2 induced by LPA. A SV40 MES13 cells were pretreated with A6730 (Akt1/2 kinase inhibitor, 4 μM) or Ly 294002 (PI3K inhibitor, 10 μM) for 1 h, and subsequently treated with LPA and/or ki16425 for 3 h. B SV40 MES13 cells were pretreated with NAC for 1 h and subsequently treated with LPA for 3 h. A and B The protein levels of p-Akt, ChREBP, and Smurf2 were analyzed by western blotting, quantified using ImageJ software, and normalized to those of β-actin. The data are presented as the mean ± SEM of results obtained from four independent experiments. *p < 0.05, **p < 0.01 vs. vehicle only (control); ^#p < 0.05, ^##p < 0.01 vs. LPA only