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Fig. 2 | Journal of Biomedical Science

Fig. 2

From: DYRK1A reinforces epithelial-mesenchymal transition and metastasis of hepatocellular carcinoma via cooperatively activating STAT3 and SMAD

Fig. 2

DYRK1A depletion attenuates the metastatic and invasion abilities of HCC cells. a HCC cells were incubated with DYRK1A siRNA or control siRNA for 48 h, and western blot analysis was performed. b and c The migration and invasion abilities of HCC cells incubated with DYRK1A siRNA or control siRNA for 48 h were assessed. d HepG2 cells were incubated with harmine and were then cultured in the upper compartment of Transwell chambers for 24 h. Then, the cells were stained with crystal violet solution. e and f A scratch was made in the HCC cell monolayer using a sterile pipette tip, and the monolayer was then washed with PBS. Images were acquired under a microscope. g and h DYRK1A-depleted and parental HCC cells were intravenously injected into nude mice (n = 6). Sixty days after injection, lung tissues and other visceral organs were excised from mice in different groups. Tissues were stained with H&E

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