Fig. 7

DYRK1A enhances EMT and the metastatic potential of HCC cells by activating STAT3. a The coexpression data were acquired from GEPIA (gepia.cancer-pku.cn). Gene: DYRK1A; The Cancer Genome Atlas Tumour: liver HCC. b The interaction between DYRK1A and STAT3 was verified by immunoprecipitation. c The levels of p-STAT3 were determined by western blot analysis in HCC cells transfected with DYRK1A siRNA. d HCC cells were incubated with harmine for 24 h, and western blot analysis was performed. e The levels of p-STAT3 in HCC cells with or without DYRK1A overexpression are shown. f The levels of the indicated proteins were determined in cells transfected with the DYRK1A overexpression plasmid or STAT3 siRNA for 48 h. g and h HCC cells were incubated with DYRK1A siRNA or the STAT3 overexpression plasmid for 48 h, and the cell migration and invasion abilities were assessed