Bioengineering in salivary gland regeneration

Hajiabbas, Maryam; D’Agostino, Claudia; Simińska-Stanny, Julia; Tran, Simon D.; Shavandi, Amin; Delporte, Christine

doi:10.1186/s12929-022-00819-w

Journal of Biomedical Science

Table 2 2D scaffold-based strategies for SG tissue engineering

From: Bioengineering in salivary gland regeneration

Material	Fabrication	Cell/tissue DC^a, AC^b	Evaluated marker/protein	Evaluated/obtained SG compartment					Refs.
Material	Fabrication	Cell/tissue DC^a, AC^b	Evaluated marker/protein	A	D	M	V	N	Refs.
PLGA	ELS	SIMS^a	–		✓				[105]
PLGA/CH laminin-111	ELS, coating	SIMS^a, SMG-C10^b	AC marker: AQP5 CP markers: β-Actin, Occludin, ZO-1 CA marker: integrin-α₆	✓	✓				[191]
PLGA	ELS	SIMS^a, mSMG^a,b Par-C10^b	CA markers: FAK, Paxillin, Talin, Vinculin	✓	✓				[185]
PLGA	ELS, Photolithography	SIMS^a, Par-C10^b	AC marker: AQP5 CP markers: E-cadherin, F-Actin, Occludin	✓	✓				[195]
PLGA/Elastin	ELS, covalent conjugation	SIMS^a	CP markers: β-Actin, F-Actin, Occludin, ZO-1		✓				[197]
PLGA/PGS	core/shell ELS	SIMS^a, NIH3T3	CP markers: F-Actin, ZO-1		✓				[198]
PVA/CH/PC	Mold Casting, Evaporation	mSMG^a,b	ECM markers: Collagen type I, III			✓			[180]
AL/G4RGDS	Mold casting, Ionic CL	mSMG^a,b	–		✓				[199]
PLGA	Mold casting	hPGAC^b	AC markers: α-amylase, AQP5 CP markers: β-Actin, E-cadherin, ZO-1 CA markers: FAK, ILK, Snail, Vinculin	✓					[200]
Agarose/laminin peptides	Mold casting	hSG, SVEC4-10, hDFs	Cell attachment marker: FAK	✓			✓	✓	[201]
PA/Human plasma fibronectin	Photo CL	mSMG^a,b	AC marker: AQP5 MC marker: SMA ECM marker: collagen IV	✓	✓	✓			[186]
PEG	Micropatterning, ELS	hPECs^a,b	AC markers: α-amylase, AQP5 DC markers: KRT7, KRT18 CP markers: β-actin, E-cadherin, F-actin, Occludin, ZO-1	✓	✓				[196]
PEG	Photopatterning, ELS	hSGSCs	AC markers: α-amylase, AQP5 DC markers: KRT5, KRT7, KRT18, NHE1, SLC26C MC marker: KRT5 CP markers: E-cadherin, ZO-1 SC markers: CD90, HAS, ITGB1, KRT5, LGR5, NANOG, OCT4, POU5F1, SOX2, THY1	✓	✓				[144]
Fibrin/Laminin-111 peptides	Solidification	Par-C10^b	CP marker: ZO-1	✓					[171]
Fibrin/Laminin-111 peptides	Solidification	Par-C10^b	AC markers: AQP5, TMEM16A DC markers: KRT7 CP markers: F-actin, Na⁺/K⁺-ATPase, ZO-1	✓	✓				[202]
PC/Agarose/PCL/HA—catechol	Mold casting, Evaporation, 3D printing	mSMG^a,b	AC marker: AQP5 MC marker: SMA EnC markers: CD31 SC marker: c-Kit, CD44	✓		✓	✓		[203]

A acinar, AC acinar cells, AL alginate, CA cell attachment, CP cell polarization, CH chitosan, CL crosslinking, D ductal, DC ductal cells, EnC endothelial cell, ECM extracellular matrix, ELS elctrospinning, G4RGDS Gly-Gly-Gly-Gly-Arg-Gly-Asp, HA hyaluronic acid, hDFs human dermal fibroblasts, hPECs human primary parotid epithelial cells, hPG human parotid gland, hPGAC human primary parotid gland acinar cells, hSG human submandibular gland ductal epithelial cell line, hSGSC human single clonal salivary gland stem cells, KRT7 keratin 7, KRT18 keratin 18, M myoepithelial, MC myoepithelial cell, mSMG ex vivo mouse submandibular gland cells, N neuronal, NIH3T3 NIH 3T3 fibroblasts, PA polyacrylamide, Par-C10 immortalized rat parotid gland acinar epithelial cell line, PC polycarbonate, PCL polycaprolactone, PEG poly(ethylene glycol), PGS poly glycerol sebacate, PLGA poly (lactic-co-glycolic acid), PVA polyvinyl alcohol, SIMS immortalized adult mouse submandibular salivary gland ductal epithelial cell line, SMA smooth muscle α-actin, SMG-C10 immortalized rat submandibular gland acinar epithelial cell line, SVEC4-10 immortalized mouse lymphoid endothelial cells, SC stem cell, V vascular

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ISSN: 1423-0127

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