Bioengineering in salivary gland regeneration

Hajiabbas, Maryam; D’Agostino, Claudia; Simińska-Stanny, Julia; Tran, Simon D.; Shavandi, Amin; Delporte, Christine

doi:10.1186/s12929-022-00819-w

Journal of Biomedical Science

Table 3 3D scaffold-based strategies for SG tissue engineering

From: Bioengineering in salivary gland regeneration

Material	Fabrication	Cell/tissue DC^a; AC^b	Evaluated markers/proteins	Evaluated/obtained SG compartment					Refs.
Material	Fabrication	Cell/tissue DC^a; AC^b	Evaluated markers/proteins	A	D	M	V	N
HA, PlnDIV	Photo CL	hPECs^a,b	AC marker: α-amylase CP marker: ZO-1	✓					[72]
HA	Thiol/acrylate CL	hPG AC	SC marker: CD44, CD168	✓					[216]
HA	Thiol/acrylate CL	hPG AC	AC marker: α-amylase, β1 adrenergic, β2 adrenergic, M3 muscarinic CP markers: β-catenin, Claudin-1, E-cadherin, ZO-1	✓					[13]
HA	Thiol/acrylate CL	hS/PCs	DC marker: KRT5 MC marker: KRT14 CP markers: β-catenin, F-actin, Occludin ECM markers: collagen IV, laminin SC markers: CD44, KRT5, KRT14	✓					[26]
HA, BM peptides	Thiol/acrylate CL	hS/PCs	AC markers: α-amylase, AQP5, HTN1, MIST1, MUC7, PIP, PSP, STATH DC markers: KRT5, KRT19, TFCP2L1 MC marker: KRT14 SC markers: c-Kit, ETV4, ETV5, KRT5, KRT14, MYC	✓	✓				[111]
HA, Peptides	Mold casting, photo CL	hS/PCs	AC markers: α-amylase DC marker: KRT5, KRT19 MC marker: KRT14, SMA CA markers: integrin α₁, integrin α₅, integrin β₁, integrin β₄ ECM markers: fibronectin; laminin SC markers: KRT5, KRT14	✓	✓	✓			[217]
HA, RGDSP	Thiol/acrylate CL	hS/PCs	AC markers: α-amylase, NKCC1 DC markers: KRT7, KRT19, TFCP2L1 CP markers: β-catenin; F-actin CA markers: CTGF, CYR61, GDF-15, SERPINE1, TGF-ß1, YAP, ECM markers: fibronectin, laminin SC markers: IGF2, KRT5, KRT14	✓	✓				[218]
Fibrin, GFRMG	Solidification, CL	mPG^a,b, Par-C10^b	AC marker: α-amylase CP markers: F-actin, ZO-1	✓					[117]
PEG, peptide	Thiol/acrylate CL	mSMG^a,b	AC marker: Mist1, NKCC1 DC marker: KRT5 SC marker: KRT5	✓	✓				[104]
PEG, MMP-degradable peptide	Thiol-ene polymerization	mSMG^a,b	AC markers: AQP5, IP3R3, MIST1, NKCC1, PIP DC marker: KRT5 CP marker: ZO-1 ECM markers: Collagen IV, Laminin SC marker: KRT5	✓	✓	✓			[27]
PEG, MMP-degradable peptide	Photo CL, Microbubble technology	mSMG^a,b hPEC^a,b	AC markers: α-amylase, AQP5, Cst3, Cst10, IP3R3, Lyz2, MIST1, M3R, Muc5b, NKCC1, PIP, P2X₇, P2Y₂; Smr3a DC markers: KRT5, KRT7 MC marker: SMA CP markers: ZO-1 SC markers: KRT5	✓	✓				[219]
SF	Casting method lyophilization	rSGECs (SMG, PG)	AC marker: α-amylase ECM marker: collagen IV	✓		✓			[182]
PLL, Au³⁺/Fe³⁺ magnetic NPs	Magnetic 3D Bioprinting	hDPSC	AC markers: α-amylase, AQP5 DC marker: KRT5 MC marker: KRT14 SC markers: CD24, CD29, CD90, C-kit, KRT5, KRT14, SOX2 NC marker: ß-tubulin	✓	✓	✓		✓	[22]
dECM	Freeze drying	rSGSCs	AC markers: α-amylase DC marker: KRT5, KRT18 CP markers: Claudin-1, Claudin-3, E-cadherin, SC markers: CD44 c-Kit, c-Met, KRT5	✓	✓				[220]

AC acinar cells, BM basement membrane, c-Met met proto oncogene, CA cell attachment, CP cell polarization, CL crosslinking, D ductal, DC ductal cell, dECM decellularized extracellular matrix, ECM extracellular matrix, GFRMG growth factor reduced Matrigel, HA hyaluronic acid, hDPSc human dental pulp stem cell, hPECs human parotid epithelial cells, hPG human parotid gland, hS/PCs primary salivary human stem/progenitor cells, mPG mouse primary parotid gland cells, M myoepithelial, MC myoepithelial cell, N neuronal, NC neuronal cell, NP nanoparticles, NS-SV-AC immortalized acinar cell from human salivary gland, Par-C10 immortalized rat parotid gland acinar epithelial cell line, PG parotid gland, PLL poly l-lysin, RGDSP integrin-binding peptide, PlnDIV peptide derived from domain IV of perlecan, rSGECs rat primary salivary gland epithelial cells, rSGSCs rat SG stem/progenitor cells, SF silk fibroin, SG salivary gland, SC stem cell, SMG submandibular salivary gland, V vascular

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ISSN: 1423-0127

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